Dmitry V. KUPRASH, PhD

kuprash@online.ru

 

Leader, "Oncoimmunology" group, Laboratory of Molecular Immunology

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

32 Vavilov street, 119991 Moscow, Russia

 

EDUCATION and DEGREES:

1994, Ph.D. in Molecular Biology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.

1990, M.S. in Applied Physics and Mathematics, Chair of Molecular Biophysics, Dept. of Physical and Chemical Biology, Moscow Institute of Physics and Technology (diploma cum laude)

 

RESEARCH INTERESTS:


We have addressed the molecular mechanisms of regulation of TNF, lymphotoxin-alpha and lymphotoxin-beta genes, by studying the promoter of these genes in various cell culture systems. We also addressed the biological role of TNF, LT-alpha and LT-beta cytokines by creating and studying mice which lack these genes either completely or in a specific cell type. As part of my transcriptional regulation studies, I developed high-resolution electrophoretic mobility shift assay for NF-kappa B proteins, and characterized several NF-kappa B dependent enhancers within the TNF/LT locus. Using serological approach, we identified a number of new candidate human cancer antigens. 

SELECTED RECENT PUBLICATIONS: 

 

  1. Shebzukhov IuV, Belousov PV, Khlgatian SV, Sazykin AIu, Kuprash DV, Nedospasov SA. [Contemporary molecular-biological methods of cancer diagnosis and monitoring based on the humoral immune response to tumor-associated antigens] Mol Gen Mikrobiol Virusol. 2007;(2):3-6. Review. Russian.
  2. Belousov PV, Shezbukhov IuV, Nedospasov SA, Kuprash DV. [Onconeural antibodies as a tool for diagnosis of malignant tumors and paraneoplastic neurological disorders] Mol Gen Mikrobiol Virusol. 2007;(2):6-13. Review. Russian.
  3. Shebzukhov YV, Koroleva EP, Khlgatian SV, Belousov PV, Sazykin AY, Kadachigova TS, Pomerantseva EA, Lagarkova MA, Nedospasov SA, Kuprash DV. RAP80/UIMC1 as cancer-associated antigen: alternative splice variants and their immunogenicity. Cancer Lett. 2007 Oct 8;255(2):255-62. Epub 2007 Jun 11.
  4. Nedospasov SA, Kuprash DV. [Oncoimmunology: some fundamental problems of cancer immunotherapy] Mol Biol (Mosk). 2007 Mar-Apr;41(2):355-68. Review. Russian.
  5. Liepinsh DJ, Grivennikov SI, Klarmann KD, Lagarkova MA, Drutskaya MS, Lockett SJ, Tessarollo L, McAuliffe M, Keller JR, Kuprash DV, Nedospasov SA.  Novel Lymphotoxin Alpha (LT{alpha}) Knockout Mice with Unperturbed Tumor Necrosis Factor Expression: Reassessing LT{alpha} Biological Functions. Mol Cell Biol. 2006 Jun; 26(11):4214-25.
  6. Drutskaya MS, Ortiz M, Liepinsh DJ, Kuprash DV, Nedospasov SA, Keller JR. Inhibitory effects of tumor necrosis factor on hematopoiesis seen in vitro are translated to increased numbers of both committed and multipotent progenitors in TNF-deficient mice. Exp Hematol. 2005 Nov; 33(11):1348-56.
  7. Shebzukhov YV, Koroleva EP, Khlgatian SV, Lagarkova MA, Meshcheryakov AA, Lichinitser MR, Karbach J, Jager E, Kuprash DV, Nedospasov SA.  Humoral immune response to thymidylate synthase in colon cancer patients after 5-FU chemotherapy. Immunol Lett. 2005 Aug 15; 100(1):88-93.
  8. Shebzukhov YV, Koroleva EP, Khlgatian SV, Belousov PV, Kuz'mina KE, Radko BV, Longpre F, Lagarkova MA, Kadachigova TS, Gurova OV, Meshcheryakov AA, Lichinitser MR, Knuth A, Jager E, Kuprash DV, Nedospasov SA.  Antibody response to a non-conserved C-terminal part of human histone deacetylase 3 in colon cancer patients. Int J Cancer. 2005 Dec 10; 117(5):800-6.
  9. Kuprash DV, Tumanov AV, Liepinsh DJ, Koroleva EP, Drutskaya MS, Kruglov AA, Shakhov AN, Southon E, Murphy WJ, Tessarollo L, Grivennikov SI, Nedospasov SA. Novel tumor necrosis factor-knockout mice that lack Peyer's patches. Eur J Immunol. 2005 May; 35(5):1592-600.
  10. Grivennikov SI, Tumanov AV, Liepinsh DJ, Kruglov AA, Marakusha BI, Shakhov AN, Murakami T, Drutskaya LN, Forster I, Clausen BE, Tessarollo L, Ryffel B, Kuprash DV, Nedospasov SA. (2005) Distinct and nonredundant in vivo functions of TNF produced by t cells and macrophages/neutrophils: protective and deleterious effects. Immunity 22(1):93-104.
  11. Koroleva EP, Lagar'kova MA, Khlgatian SV, Shebzukhov IuV, Meshcheriakov AA, lichinitser MR, Nedospasov SA, Kuprash DV. (2004) [Serological study of a repertoire of human cancer antigens and autoantigens] Mol Biol (Mosk) 38(2):233-8.
  12. Tumanov A.V., Kuprash D.V., Mach J.A., Nedospasov S.A., and Chervonsky A.V. (2004). Lymphotoxin and TNF produced by B cells are dispensable for maintenance of the follicle-associated epithelium but are required for development of lymphoid follicles in the Peyer's patches. J. Immunol. 173, 86-91.
  13. Shakhov A.N., Rybtsov S.A., Tumanov A.V., Shulenin S., Dean M., Kuprash D.V., and Nedospasov S.A. (2004). SMUCKLER/TIM4 is a distinct member of TIM family expressed by stromal cells of secondary lymphoid tissues and associated with lymphotoxin signaling. Eur J Immunol 34, 494-503.
  14. Tumanov A.V., Kuprash D.V., and Nedospasov S.A. (2003). The role of lymphotoxin in development and maintenance of secondary lymphoid tissues. Cytokine Growth Factor Rev. 14, 275-288.
  15. Tumanov A.V., Grivennikov S.I., Shakhov A.N., Rybtsov S.A., Koroleva E.P., Takeda J., Nedospasov S.A., and Kuprash D.V. (2003). Dissecting the role of lymphotoxin in lymphoid organs by conditional targeting. Immunol Rev. 195, 106-116.
  16. Nedospasov S.A., Grivennikov S.I., and Kuprash D.V. (2003). Physiologic roles of members of the TNF and TNF receptor families as revealed by knockout models. In Cytokine knockouts, G.Fantuzzi, ed. (Totowa, NJ: Humana Press), pp. 439-460.
  17. Lagarkova M.A., Koroleva E.P., Kuprash D.V., Boitchenko V.E., Kashkarova U.A., Nedospasov S.A., and Shebzukhov Y.V. (2003). Evaluation of humoral response to tumor antigens using recombinant expression-based serological mini-arrays (SMARTA). Immunol. Lett. 85, 71-74.
  18. Tumanov A.V., Kuprash D.V., Lagarkova M.A., Grivennikov S.I., Abe K., Shakhov A.N., Drutskaya L.N., Stewart C., Chervonsky A.V., and Nedospasov S.A. (2002). Distinct role of surface lymphotoxin expressed by B cells in the organization of secondary lymphoid tissues. Immunity 17, 239-250.
  19. Kuprash D.V., Alimzhanov M.B., Tumanov A.V., Grivennikov S.I., Shakhov A.N., Drutskaya L.N., Marino M.W., Turetskaya R.L., Anderson A.O., Rajewsky K., Pfeffer K., and Nedospasov S.A. (2002). Redundancy in Tumor Necrosis Factor (TNF) and Lymphotoxin (LT) Signaling In Vivo: Mice with Inactivation of the Entire TNF/LT Locus versus Single-Knockout Mice. Mol. Cell Biol. 22, 8626-8634.
  20. Kuprash D.V., Boitchenko V.E., Yarovinsky F.O., Rice N.R., Nordheim A., Ruhlmann A., and Nedospasov S.A. (2002). Cyclosporin A blocks the expression of lymphotoxin alpha, but not lymphotoxin beta, in human peripheral blood mononuclear cells. Blood 100, 1721-1727.
  21. Koroleva E.P., Lagarkova M.A., Mesheryakov A.A., Scanlan M.J., Old L.J., Nedospasov S.A., and Kuprash D.V. (2002). Serological identification of antigens associated with renal cell carcinoma. Russ. J. Immunol. 7, 229-238.
  22. Kuprash D.V., Alimzhanov M.B., Tumanov A.V., Anderson A.O., Pfeffer K., and Nedospasov S.A. (1999). TNF and lymphotoxin beta cooperate in the maintenance of secondary lymphoid tissue microarchitecture but not in the development of lymph nodes. J Immunol. 163, 6575-6580.
  23. Kuprash  D.V., Udalova I.A., Turetskaya R.L., Kwiatkowski D., Rice N.R., and Nedospasov S.A. (1999). Similarities and differences between human and murine TNF promoters in their response to lipopolysaccharide. J Immunol 162, 4045-4052.